Me: Everytime I got time on the confocal

I understand, and I definitely don’t fault anyone who likes the show or stuck through it. I just feel like too many shows I’ve been watching lately hasn’t been worth the slog to get through, and it’s been killing my excitement for franchises.

For example I haven’t watched house of the dragon because game of thrones rushed ending, Picard season 3 because of season 2, and I fell off the marvel train after Wanda vision, falcon and winter soldier, and she hulks endings, all shows that either ending in nothing burgers or wtf I guess we need to end somehow.

I don’t think anyone who sat down and watched through the first 3 episodes can honestly say the show is being review bombed. Im enjoyed Ashoka and Boba Fett and I honestly have no interest in wasting my time finishing the show.

It’s like the nothing burgers of the Disney star wars comics. The Darth Vader series was great, and I love the idea of bleeding light sabers red. But everyone other series leading into the last Jedi was a waste of time, and I bought and read every single one.

I’ve been buying used 12tb HGST from Amazon for $80.

Lol yeh but it’s partially funded by money saved from canceled subscriptions.

I’m a noob, I started 2 months ago with immich and tailscale. Now I have an unraid server, it’s a slippery slope.

Pretty good description. Few extra points. -you don’t need to don’t need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert. -these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.

For what is possibly wrong with the top gel.

• the wavy line in some bands indicates uneven current, this can be due to sample composition before loading the gel, like having cellular debris along with your DNA or a mismatch between the buffer used to make the gel and the running buffer.

-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you’ll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it’s not pushed in to gel evenly, or it was run to fast.

Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don’t know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.

Most definitely and probably be missing some limb by now.

Star wars has been effectively marketing me lightsabers for years, and I can’t even carve a turkey with mine.

I wish they would kill the MCU and just start over comic book style.

Can we do radio shack from the 80s early 90s over the cellphone retailer they turned into before death.